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code for colocalized synaptic puncta in astrocyte territories  (MathWorks Inc)


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    MathWorks Inc code for colocalized synaptic puncta in astrocyte territories
    (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized <t>puncta</t> density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .
    Code For Colocalized Synaptic Puncta In Astrocyte Territories, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/code for colocalized synaptic puncta in astrocyte territories/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    code for colocalized synaptic puncta in astrocyte territories - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development"

    Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

    Journal: Cell reports

    doi: 10.1016/j.celrep.2022.110416

    (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized puncta density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .
    Figure Legend Snippet: (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized puncta density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .

    Techniques Used: Immunostaining

    (A) Schematic of PALE with CMV-shRNA-tGFP and pCAG-mTdT in postnatal mice. (B) P21 PALE astrocytes from deep layers of scramble-shRNA and Lrig1-shRNA groups. Scale bar, 10 μm. (C) Sholl analysis of scramble-shRNA and Lrig1-shRNA astrocytes. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; each group includes 26–35 cells (z stack). (D and E) Immunostaining for VGluT1/PSD95 (D) and VGluT2/PSD95 (E) excitatory synapses in P21 scr-shRNA and Sparc-shRNA groups. Scale bar, 10 μm. (F and G) Quantification of co-localized puncta density in tdTomato + astrocyte domains. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; Sparc-shRNA: n = 6 mice; Il33-shRNA: n = 7 mice; each V1 group includes 29–33 cells (z stack) and each V2 group includes 23–26 cells. Data represent mean ± SEM; statistical analyses were multiple t test (C) or one-way ANOVA (F, G). See also .
    Figure Legend Snippet: (A) Schematic of PALE with CMV-shRNA-tGFP and pCAG-mTdT in postnatal mice. (B) P21 PALE astrocytes from deep layers of scramble-shRNA and Lrig1-shRNA groups. Scale bar, 10 μm. (C) Sholl analysis of scramble-shRNA and Lrig1-shRNA astrocytes. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; each group includes 26–35 cells (z stack). (D and E) Immunostaining for VGluT1/PSD95 (D) and VGluT2/PSD95 (E) excitatory synapses in P21 scr-shRNA and Sparc-shRNA groups. Scale bar, 10 μm. (F and G) Quantification of co-localized puncta density in tdTomato + astrocyte domains. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; Sparc-shRNA: n = 6 mice; Il33-shRNA: n = 7 mice; each V1 group includes 29–33 cells (z stack) and each V2 group includes 23–26 cells. Data represent mean ± SEM; statistical analyses were multiple t test (C) or one-way ANOVA (F, G). See also .

    Techniques Used: shRNA, Immunostaining

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, RNAscope, Multiplex Assay, Real-time Polymerase Chain Reaction, In Situ Hybridization, Software



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    code for colocalized synaptic puncta in astrocyte territories  (MathWorks Inc)
    90
    MathWorks Inc code for colocalized synaptic puncta in astrocyte territories
    (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized <t>puncta</t> density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .
    Code For Colocalized Synaptic Puncta In Astrocyte Territories, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/code for colocalized synaptic puncta in astrocyte territories/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    code for colocalized synaptic puncta in astrocyte territories - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized puncta density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .

    Journal: Cell reports

    Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

    doi: 10.1016/j.celrep.2022.110416

    Figure Lengend Snippet: (A and C) Immunostaining for VGluT1/PSD95 (A) and VGluT2/PSD95 excitatory synapses (C) in layers II/III and layer V of WT and Ptch1cKO cortical astrocytes. Scale bar, 5 μm. (B and D) Quantification of co-localized puncta density in tdTomato + astrocyte domains per mm 2 . N = 4 mice for each condition. (E–G) Recordings of mEPSC from layer V neurons in WT and Ptch1cKO. N = 3 mice for each condition (WT: n = 20 cells; KO: n = 22 cells). (H) Recordings of evoked EPSC from layer V neurons of WT and Ptch1cKO cortex. N = 4 mice for each condition (WT: n = 20 cells; KO: 15 cells). Data represent mean ± SEM; statistical analyses were Welch’s t test (B, D, F, G) or multiple t test (H). *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also .

    Article Snippet: MATLAB code for colocalized synaptic puncta in astrocyte territories , This manuscript , https://doi.org/10.5281/zenodo.5879992.

    Techniques: Immunostaining

    (A) Schematic of PALE with CMV-shRNA-tGFP and pCAG-mTdT in postnatal mice. (B) P21 PALE astrocytes from deep layers of scramble-shRNA and Lrig1-shRNA groups. Scale bar, 10 μm. (C) Sholl analysis of scramble-shRNA and Lrig1-shRNA astrocytes. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; each group includes 26–35 cells (z stack). (D and E) Immunostaining for VGluT1/PSD95 (D) and VGluT2/PSD95 (E) excitatory synapses in P21 scr-shRNA and Sparc-shRNA groups. Scale bar, 10 μm. (F and G) Quantification of co-localized puncta density in tdTomato + astrocyte domains. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; Sparc-shRNA: n = 6 mice; Il33-shRNA: n = 7 mice; each V1 group includes 29–33 cells (z stack) and each V2 group includes 23–26 cells. Data represent mean ± SEM; statistical analyses were multiple t test (C) or one-way ANOVA (F, G). See also .

    Journal: Cell reports

    Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

    doi: 10.1016/j.celrep.2022.110416

    Figure Lengend Snippet: (A) Schematic of PALE with CMV-shRNA-tGFP and pCAG-mTdT in postnatal mice. (B) P21 PALE astrocytes from deep layers of scramble-shRNA and Lrig1-shRNA groups. Scale bar, 10 μm. (C) Sholl analysis of scramble-shRNA and Lrig1-shRNA astrocytes. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; each group includes 26–35 cells (z stack). (D and E) Immunostaining for VGluT1/PSD95 (D) and VGluT2/PSD95 (E) excitatory synapses in P21 scr-shRNA and Sparc-shRNA groups. Scale bar, 10 μm. (F and G) Quantification of co-localized puncta density in tdTomato + astrocyte domains. scr-shRNA: n = 4 mice; Lrig1-shRNA: n = 6 mice; Sparc-shRNA: n = 6 mice; Il33-shRNA: n = 7 mice; each V1 group includes 29–33 cells (z stack) and each V2 group includes 23–26 cells. Data represent mean ± SEM; statistical analyses were multiple t test (C) or one-way ANOVA (F, G). See also .

    Article Snippet: MATLAB code for colocalized synaptic puncta in astrocyte territories , This manuscript , https://doi.org/10.5281/zenodo.5879992.

    Techniques: shRNA, Immunostaining

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

    doi: 10.1016/j.celrep.2022.110416

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: MATLAB code for colocalized synaptic puncta in astrocyte territories , This manuscript , https://doi.org/10.5281/zenodo.5879992.

    Techniques: Recombinant, RNAscope, Multiplex Assay, Real-time Polymerase Chain Reaction, In Situ Hybridization, Software